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Marine microbial eukaryotes are underexplored, yet are known to be important to the marine ecosystem, being part of the complex food web as well as contributing to nutrient cycling within the ocean. Marine microbial eukaryotes found in seagrass beds are undoubtedly important to seagrass ecosystem health and overall marine ecosystem processes.   We propose to perform a global census of microbial eukaryotes found in association with the leaves, roots, and sediment of the seagrass _Zostera marina_. We will then expand our investigation to census the microbial eukaryotes found in association with plants across the Order Alismatales, which includes three independent lineages of seagrasses. Concurrently with the afformentioned censuses, we will establish a culture collection of microbial eukaryotes found associated with _Zostera marina_ from Bodega Bay, California. We are uniquely positioned to be successful at the proposed research; using funds provided by the Gordon and Betty Moore Foundation, we have already established a program to explore bacterial diversity within seagrass beds, and have completed the majority of field work and formed ongoing  collaborations with other seagrass researchers from both the ZEN group Zostera Experimental Network (ZEN)  and other research institutions.