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-   Seagrass beds are hotspots of diversity (cite macrobe studies) and provide homes to many rare/endemic species. Likely also harbor much unexplored microbial diversity. Area of marine microbial eukaryotes is not well studied, and the diversity of microbial eukaryotes in general is undercharacterized. We aim to identify and characterize microeukaryotic diversity within seagrass beds. Microeukaryotes are important in ocean ecosystems (predator, prey, biogeochemical cycling of nutrients etc.).   ###Rationale and Significance (3/4 page)  1. briefly cite literature that will support, in greater detail, the eistence of the knowledge gap or need  2. additional detail on why gap is a problem  3. describe why what you want to do is creative, original, and if credible, potentially transformative  4. make explicit statement about what your contribution will be  5. fully italicized statement of significance: why will your contribution have a positive impact on something that is important to NSF and its proirities  6. conclude with tangible benefits that are likely to accrue through application of new knowledge  make sure to mention this somewhere: http://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12282/full  -looked at microbial community in biofilm of Enhalus (both bacteria and eukaryotes--18s--) Their study suggests   that seagrasses could be vectors for coral pathogens    (If hypothesis-driven)  ###Hypotheses (1/2 page)  1. write specific aims, followed by the hypotheses that will be driving them  - "To test overarching hypothesis - mentioned above - we propose the following specific aims"  - list aims, each of which should test a readily identifiable part of the central hypothesis  (If not hypothesis-driven)  ###Research Questions (1/2 page)  1. a list of questions that, when answered, will lead to the attainment of the overall objective  Examine phylogeographic signal of Zostera marina-associated microeukaryotes -- is everything everywhere? Do species follow geographic patterns?   Examine multi species patterns of seagrass associated microeukaryotes. Phylogenetic signal? What about in seagrass patches where there are multiple species of seagrass co-occurring -- do they have the same associations? different?   Create cultures of Zostera associated microeukaryotes from Bodega Bay -- make publicly available.   ###Research Approach (1 3/4 pages)  1. describe what you want to do!  2. offer small amount of preliminary data to demonstrate feasibility  3. if you have room, present approach under each research aim  4. each aim should be addressed fully enough so that reviewers have some idea of what you would present in a full proposal  primers: F566-R1200 650 bp V4/V5, 82F-516R 480 bp V2/V3, universal euk primers targetting V4/V9 regions  Zostera marina aim: ZEN samples (leaf, root, sediment) for 18s (see above primer list).   Multi species: same methods as Zmarina  Culture collections: Use Zostera from Bodega Bay (maybe try from different locations, different genotypes, etc.).  *highlight that we have experience doing all of these types of things, and leverage ZEN contacts and others willing to collaborate on sampling. Prelim data on fungi, cpDNA data from ZEN?   ###Broader Impacts (>1/2 page)  how will this benefit society or accomplish NSF's desired societal outcomes?  characterization of biodiversity in understudied area  publicly available data (sequence, blog, cultures)  large outreach component