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Our lab has an excellent track record with science communication, citizen science and outreach. We also have a history of fostering and encouraging undergraduate research and many of our previous undergraduates have published first author papers (examples: \cite{Lee_2015} \cite{Betts_2015} \cite{Flanagan_2013} \cite{Dunitz_2014} \cite{Holland_Moritz_2014}). The project we are proposing will include a large outreach component. We plan to have undergraduate researchers perform parts of Aim 3 including culturing, microscopy and sequence analysis as documented in our published workflow \cite{Dunitz_2015}. We have previously had success mentoring high school students for summer research through the Young Scholar's Program at our university and would like to include such students in Aim 3 as well. We have an ongoing collaboration with a local AP Biology class at Western Sierra Collegiate Academy, where students have performed experiments using Winogradsky columns built from seagrass bed sediment. We plan to utilize this connection again to make modified hay infusion enrichments (http://materialscience.uoregon.edu/ttsem/curriculum%20to%20share/Microbes%20from%20Hay%20lab.pdf) using seagrass so that they will be able to isolate and culture microeukaryotes for inclusion in our culture collection. We have plans to expand this outreach effort in collaboration with Liza Conrad at Eckerd College in Saint Petersburg, Florida.  Our This grant will help support  grad students who  lead workshops at UC Berkeley's Expanding Your Horizons (EYH) event for 6th-9th grade girls every year on basic genetics and phylogenomics/evolutionary trees. They also volunteer annually at the Sacramento EYH event, and are actively involved with a variety of groups at UC Davis including two focused on facilitating women in science, Sisters In Science (SiS) and Women In Science (WiSci), and another focused on local outreach, the Davis Science Collective.