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Cluster description: http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503666&org=DEB&from=home  ##Overview  Identify Theh broad goal of this project is to identify  and characterize microbial eukaryotes associated with seagrass beds. Microorganisms, and specifically marine microbial eukaryotes, represent an underexplored area of diversity. Microbial eukaryotes are known to be important on a number of trophic levels in the marine system **CITE**, and microbial eukaryotes found in seagrass  beds (specifically Zostera marina). likely contribute to their tremendous biodiversity and roles as important players in nutrient cycling and carbon sequestration in the oceans. We will use a combination of sequencing and culturing techniques to (1) characterize microeukaryotes in a global census of the seagrass _Zostera marina_, (2) Explore microbial eukaryotic diversity across the Order Alismatales, including the 3 separate lineages of seagrasses and their freshwater and brackish relatives, and (3) Create a publicly available culture collection of microbial eukaryotes from _Zostera marina_ samples from Bodega Bay, CA.  Samples: ZEN data (not H20), sediment, leaves, roots + scrapings  Challanges: uncharacterized diversity (bad databases), plant 18S