deletions | additions       diff --git a/Research_Approach_1_3_4__.md b/Research_Approach_1_3_4__.md  index cd29a87..e77efc4 100644  --- a/Research_Approach_1_3_4__.md  +++ b/Research_Approach_1_3_4__.md 
...
-describe CA collections + Austrailia + ZEN ??  We are currently utilizing our ZEN contacts to collect leaves, roots, and sediment from plants from the Order Alismatales and have plans to amplify and sequence the 16S ribosomal RNA genes of the microbial communities in these samples. Our goal is to have samples from a minimum of three representative species from each of the three seagrass lineages, three of the closest freshwater relatives for each seagrass lineage and two terrestrial outgroups for a minimum of 20 plant species. However, based on collections from our collaborators as well as local collections performed by our lab, it is very likely that we will supercede our minimum collection goal for seagrass and freshwater species. We currently have (?????) 414 samples from (??)  plant species samples in hand. For each plant species, 5 biological replicates of root, leaf and rhizosphere sediment will be collected at a minimum of three geographic locations. _Molecular Biology/Bioinformatic Anaylsis_:  The V4 and V9 variable regions of the 18S ribosomal RNA gene will be targetted for amplification as in Aim 1 and the same general bionifornmatic pipeline and statistical analyses methods will be performed. Addtional statistical analyses will be implemented to determine if distinct microbial communities are found in association with different host plants.