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_Fungal Isolation/Culturing_: A variety of media recipes found previously successful at isolating seagrass-associated fungi will be used here initially. These include potato dextrose agar, malt-dextrose agar, glucose peptone yeast agar and cornmeal agar, with all media being supplemented with salt and antibiotics \cite{Torta_2014}\cite{Vohn_k_2015}\cite{Panno_2013}. Media will also be made using Z. marina as the carbon source as was done by Torta el al \cite{Torta_2014} using the seagrass, Posidonia oceanica.   _Amoeba Isolation/Culturing_: To enrich for amoeba, samples will be inoculated onto plates containing ???? media(1 mL 0.5 M MOPS buffer, 0.1 g yeast extract, 0.1 g tryptone, 15 g agar and 1X seawater base to make 1L)  with a marine bacterium as a food source. Upon formation, plaques will be picked from the plate and inoculated into flasks containing approximately 10 mL of liquid media (no agar added). (??) media.  Flasks will be monitored for amoeba growth. -algae + protist media recipes  http://www.ccap.ac.uk/pdfrecipes.htm