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-describe CA collections + Austrailia + ZEN ??  We are currently utilizing our ZEN contacts to collect leaves, roots, and sediment fromaround the world from  plants from the Order Alismatales and have plans to amplify and sequence the 16S ribosomal RNA genes of the microbial communities in these samples. Our goal is to have samples from a minimum of three representative species from each of the three seagrass lineages, three of the closest freshwater relatives for each seagrass lineage and two terrestrial outgroups for a minimum of 20 species. However, based on collections from our collaborators as well as local collections, it is very likely that we will supercede our minimum collection goal for seagrass and freshwater species. We currently have (?????) plant species samples in hand. For each plant species, roots, leaves and rhizosphere sediment will be collected in quintuplicate per species per geographic location with a minimum of three geographic locations per species. _Molecular Biology/Bioinformatic Anaylsis_:  The V4 and V9 variable regions of the 18S ribosomal RNA gene will be targetted for amplification as in Aim 1 and the same general bionifornmatic pipeline and statistical analyses methods will be performed. Addtional statistical analyses will be implemented to determine if distinct microbial communities are found on different host plants.