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The proposed project would result in an increase in the number of marine microeukaryotic ribsomal RNA sequences in open online databases as well as increase the number of microeukaryotic cultures available to researchers around the world for characterization and experimentation. Addtionally, this project would expand our knowledge of the tree of life and help with current research investigating the topology of this tree.   To achieve our goals, we will first perform a global census of microbial eukaryotes found in association with the leaves, roots, and sediment of the seagrass _Zostera marina_. We will then expand our investigation to census the microbial eukaryotes found in association with leaves, roots, and sediment of the plants across  Order Alismatales, which includes three, independent lineages of seagrasses. Concurrently with the afformentioned censuses, we will establish a culture collection of microbial eukaryotes found associated with _Zostera marina_ from Bodega Bay, California.