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Microorganisms comprise the majority of diversity on Earth. Traditionally classified using morphological approaches, the advent of sequence data has dramatically altered our views of microbial evolution and diversity. Specifically, high throughput sequencing technologies have enabled us to explore multiple genes and genomes from microorganisms, giving us insight into genome complexity and function in these organisms. As a result microbial ecologists are finding themselves in uncharted territory as they analyze large data sets full of "unclassified" organisms, and it has become clear that microorganisms are much more diverse than previously thought. Our goal with this proposal is to increase the overall knowledge of the true diversity of microbial eukaryotes by identifying and culturing microeukaryotes from seagrass beds.  Seagrasses are the only group of fully-submerged marine angiosperms, and are globally distributed along coastlines of every continent except Antarctica \cite{Costanza_1997}. They form dense meadows in shallow waters along the coastline, providing critical habitat for many marine species \cite{Harborne_2006}. These meadows harbor tremendous amounts of biodiversity, providing homes to many rare/endemic macroogramisms and are critical in biogeochemical cycling in the ocean system \cite{Orth_2006}. (Is there any evidence that tropical rain forests have more microbial diversity than other locations? if so, could use as justification?? closet find so far is this \cite{Fierer_2012} maybe http://www.ncbi.nlm.nih.gov/pubmed/23832301 or http://www.labmanager.com/news/2009/07/amazon-rainforest-microbial-observatory-to-catalog-study-soils#.VqKKS1MrKHo ??) Although they occupy a very small percentage of area on Earth \cite{Costanza_1997} they are one of the most important carbon sinks, sequestering carbon 35X faster than Tropical Rainforests \cite{McLeod_2011} \cite{Mcleod_2011}  and storing this carbon for millennia if left undisturbed \cite{Macreadie_2012} \cite{Mateo_1997} \cite{Serrano_2012}. something about carbon sink, important etc., all of these reasons make them excellent candidates etc. know how important microbial euks are to biogeo ocean cycles