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###Research Approach (1 3/4 pages)  ####**Aim 1: A global census of microbial eukaryotes found in association with leaves, roots, and sediment of the seagrass Zostera marina.** We have, in hand, DNA that has been extracted from 400 samples of _Zostera marina_ leaves, roots, and sediment (ZEN samples). These samples include 3 biological replicates of each sample type. Samples were take from two seagrass beds at each of 22 sites in northern Europe, the Mediterranean, Japan, Australia, Central America, and both coasts of the United States. We have successfully amplified and sequenced the 16S ribosomal RNA genes of the microbial communities in these samples. We have demonstrated success amplifying, sequencing, and analyzing eukaryotic microbial loci (fungal ITS) from samples prepared in the same way as the ZEN samples. We also have a wealth of metadata associated with each site: 109 measurements of plant traits (including biomass, productivity, and microsatellite loci), water and sediment characteristics, and abundances of invertebrate grazers and predator species. We will use universal microbial eukaryotic primers, targeting the V4 and V9 variable regions of the 18S ribosomal RNA gene. These regions are standardly used in such inventories and are known to be informative at the (??? taxonomic) level.