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####**Aim 2: A global census microbial eukaryotes found in association with leaves, roots, and sediment of the Order Alismatales, which includes three, independent lineages of seagrasses**  ####**Aim 3: Establish culture collection of microbial eukaryotes associated with Zostera marina in Bodega Bay, California.**  Fungal Isolation. A variety of media recipes found previously successful at isolating seagrass-associated fungi will be used here. These include potato dextrose agar, malt-dextrose agar, glucose peptone yeast agar and cornmeal agar, with all media being supplemented with salt and antibiotics (27, 28, 42). Media will also be made using Z. marina as the carbon source as was done by Torta el al (27) using the seagrass, Posidonia oceanica.  primers: F566-R1200 650 bp V4/V5, 82F-516R 480 bp V2/V3, universal euk primers targetting V4/V9 regions