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##Overall taxonomic diversity of ISS surfaces and comparison to previous high-throughput 16SrRNA study  After filtering chimeric and eukaryotic sequences from the data, the number of sequences per surface sampled ranged from 26,221 - 76,656. Open-reference clustering at 97% similarity resulted in 12,554 OTUs (OTU is a proxy for microbial "species".) This exceeds the number of species observed by Venkateswaran _et al._ 2014, which is not surprising, given the increased sampling depth in this study (~1 million versus ~ 50,000 high-quality sequences.) Our study also had three notable, qualitative differences from Venkateswaran at et  al. 2014. First, in their study, more than 90% of all sequences were assigned to 4 bacterial genera (_Corynebacterium_, _Propionibacterium_, _Staphylococcus_, and _Streptococcus_), while in the study here, they comprised only 24% of the data (9.6%, 0.05%, 10.7%, and 3.6%, respectively). Second, Venkateswaran at et  al. found no evidence of archaea in their samples, even when interrogating with archaeal-specific primers, but we did find evidence for a very low-abundance archaeal presence (2335 sequences, from three archaeal phyla). Finally, despite the fact that Venkateswaran at et  al. were able to culture many spore-forming organisms from their samples, they observed no sequence data from putative spore-forming organisms. However, a large percentage of sequences in our study are from spore-forming genera: 20.9% _Bacillus_ and 9.6% _Clostridium_. These differences are potentially due to differences in PCR primers and/or DNA extraction method, both of which have known taxonomic biases \cite{Brooks_2015}. The 19 most abundant orders found in our study represent 93.8% of the data (Figure PieChart). Within each of these 19 orders, the most abundant genus found in our samples tends to be human-associated (Table habitat). This is not surprising, as the only source of microbial influx is via occasional crew and cargo deliveries aboard spacecraft that have been stringently cleaned to avoid microbial contamination.