deletions | additions       diff --git a/Methods.md b/Methods.md  index ae9d8c7..fbfa473 100644  --- a/Methods.md  +++ b/Methods.md 
...
16S rRNA gene (515F-806R) was amplified with region-specific primers that included   the Illumina flowcell adapter sequences and a twelve base barcode sequence. Each 25 ul   PCR reaction contained the following: 12 ul of PCR water certified DNA-free (MO BIO),   10ul 10 ul  of 1x 5 Prime HotMasterMix (5 Prime), 1 ul of Forward Primer (5uM concentration, 200pM 200 pM  final), 1 ul of Golay Barcode Tagged Reverse Primer (5uM concentration, 200pM final), and 1 ul of template DNA. The conditions for PCR were as follows: 94°C for 3   minutes to denature the DNA, with 35 cycles at 94 °C for 45 s, 50 °C for 60 s, and 72 °C   for 90 s, with a final extension of 10 min at 72 °C to ensure complete amplification.