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Jenna M. Lang deleted file Introduction.md
over 9 years ago
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##Sample aquisition and processing
Astronauts were instructed to swab 15 surfaces on the International Space Station (see Figure 1 for sampling locations and complete crew instructions). These surfaces were chosen in an attempt to sample surfaces analogous to those sampled in the Wildlife of Our Homes project \cite{23717552}. Wildlife of Our Homes was a Citizen Science project for which over 1000 volunteers swabbed nine surfaces in their homes: kitchen cutting board, kitchen counter, a shelf inside a refrigerator, toilet seat, pillowcase, exterior handle of the main door into the house, television screen, the upper door trim on the outside surface of an exterior door, and the upper door trim on an interior door. We were not granted access to all corresponding surfaces aboard the ISS. The kitchen surfaces aboard the ISS are in the Russian module, which we did not have permission to access, the toilet seat was deemed inappropriate due to ???, and the exterior surfaces are accessible only via an "Extra-vehicular Activity" (Space Walk,) which was not requested for this experiment.
Upon completion of the swabbing on April/May ??? (include link to video of actual swabbing?) All swabs were stored at -80 deg C, until transfer to the SpaceX Dragon spacecraft, which re-entered the Earth's atmosphere and splashed down in the Pacific Ocean at 12:05pm PT on May 18, 2014. Samples were transferred by boat to ???, transferred to a cooler with dry ice, and shipped to blah, blah, blah, EMP.
Insert description of DNA extraction, PCR, sequencing.
##Bioinformatic Analysis
Demultiplex, QIIME, blah,blah, blah
