deletions | additions       diff --git a/methods.tex b/methods.tex  index ecd04f7..57005a0 100644  --- a/methods.tex  +++ b/methods.tex 
...
\subsection{Experimental Design}  Basic setup of the experiment. Which genotypes and why, what kind of food, which developmental stage went up in the rocket, how many went up, how many came down alive, etc...  \subsection{Sample Collection}  Description of the dissections. Tissue samples were transferred directly into the Bead Tubes provided in the MoBio Power Soil Microbial DNA Isolation Kit (MoBio Laboratories, Inc.), and stored at -20degC until the DNA extractions were performed. \subsection{16S rDNA amplicon library construction and sequencing}  DNA was extracted from all samples with the MoBio Power Soil kit, according to the manufacturer's instructions, with a final elution of 50uL. The 16S rRNA genes were targeted for amplification via PCR, using primer constructs that consisted of the "universal" bacterial primers 515F and 806R, both modified by the addition of Illumina adaptor and an 8bp barcode sequence. (All primer sequences used for this study are shown in table XXX). The PCR amplification protocol included a 3 minute denaturation step at 94degC, 30 cycles of (30sec at 94degC, 45sec at 55degC, 90sec at 72degC), and a final 10min extension step of 72degC. PCR reactions were visualized on an agarose gel and purified using AMPure Beads (Agilent Technologies, Inc.) Purified PCR products were quantified with the Qubit® 2.0 Fluorometer (Life Technologies) HS dsDNA assay. All samples were pooled in eqiumolar ratios prior to sequencing on the Illumina MiSeq sequencer.   \subsection{Sequence Processing}