deletions | additions       diff --git a/Methods.md b/Methods.md  index bd9bfb5..cfd7ee8 100644  --- a/Methods.md  +++ b/Methods.md 
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the Illumina flowcell adapter sequences and a twelve base barcode sequence. Each 25 ul   PCR reaction contained the following: 12 ul of PCR water certified DNA-free (MO BIO),   10 ul of 1x 5 Prime HotMasterMix (5 Prime), 1 ul of Forward Primer (5 uM concentration,   200 pM final), 1 ul of Golay Barcode Tagged Reverse Primer (5uM (5 uM  concentration, 200pM 200 pM  final), and 1 ul of template DNA. The conditions for PCR were as follows: 94°C for 3   minutes to denature the DNA, with 35 cycles at 94 °C for 45 s, 50 °C for 60 s, and 72 °C   for 90 s, with a final extension of 10 min at 72 °C to ensure complete amplification.