Authorea

David Coil added Comparison to previous work.tex over 9 years ago

Commit id: 182c0a0e2569c89417e0fe70b6857703d54dddb0

deletions | additions

Castro et al 2004. Using culturing, 16S PCR (not next-gen), and DNA fingerprinting to look at initial colonization of the ISS by microbes. Cite in intro, not really relevant to our study. 10.1007/s00248-003-1030-y Kawamura et al 2001. Using culturing and a bit of taxa-specific PCR to assay microbes on Mir. Cite in intro. 10.1111/j.1348-0421.2001.tb01321.x/pdf Pearson 2001. "Microbial contamination in spacecraft" is a review on what was known to date and how that information was used in designing the ISS. Cite in intro. Bernardini 2005. Microbial survey of ISS cooling system. Can't find the paper, need to dig it up and see if it's worthwhile. Conference proceedings. Moissel 2007. Used 16S surveys (full-length primers followed by TOPO cloning) to examine the earthbound ISS simulator which attempts to replicate the conditions on the ISS as much as possible. Cite in intro? http://dx.doi.org/10.1017/S1473550406003533 Venkateswaran et al 2014. This claims to be the first molecular method survey of the microbial communities present on the ISS. Used ATP and PMA tests coupled with 454 sequencing and culturing. Did bacteria, fungi, and archaea. Makes a big deal about viability issues and disconnect between culturing (lot of spore formers) and sequencing (many other taxa, little spore formers). We need to address this (do we see spore formers, e.g. Bacillus in our data?). Probably just an issue of DNA extraction (is this common in built environments). -only dust (i.e. no spatial resolution). - -Only 51,000 sequences for analysis after filtering. -more than 90 percent of reads from Corneybacterium, Propionibacterium, Staphylococcus, and Streptococcus (is this similar for us or not?) -no archaea observed (did we see any?)