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Jenna M. Lang edited Comparison to previous work.tex
about 9 years ago
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#Comparison to previous work
Kawamura et al 2001. Using culturing and a bit of taxa-specific PCR to assay microbes on Mir. Cite in intro. 10.1111/j.1348-0421.2001.tb01321.x/pdf
Moissel 2007. Used 16S surveys (full-length primers followed by TOPO cloning) to examine the earthbound ISS simulator which attempts to replicate the conditions on the ISS as much as possible. Cite in intro? http://dx.doi.org/10.1017/S1473550406003533
Venkateswaran et al 2014. This claims to be the first molecular method survey of the microbial communities present on the ISS. Used ATP and PMA tests coupled with 454 sequencing and culturing. Did bacteria, fungi, and archaea. Makes a big deal about viability issues and disconnect between culturing (lot of spore formers) and sequencing (many other taxa, little spore formers). We need to address this (do we see spore formers, e.g. Bacillus in our data?). Probably just an issue of DNA extraction (is this common in built environments).
-only dust (i.e. no spatial resolution).
-Only 51,000 sequences for analysis after filtering.
-no archaea observed (did we see any?)
