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15. Crew Choice Surface: Audio Terminal Unit (telephone) hand held push-to-talk microphone located in the starboard portion of the Harmony module (Node 2).
## Swabbing instructions:
1. _1. Setup Node-2 Camcorder to capture NanoRacks surface swab Ops throughout the US LAB.
2. _
_2. Retrieve a clean NanoRacks Swab
Kit.
Move Kit._
_Move to ISS location listed on NanoRacks Swab Kit label.
3. _
_3. Remove cotton swab from NanoRacks Swab Kit, being careful not to touch the cotton swab tip to avoid contamination.
4. _
_4. Rub cotton swab vigorously against designated surface. Spin and turn the swab to ensure maximum sample collection.
5. _
_5. Return cotton swab to NanoRacks Swab Kit and press to close (squeeze excess air out of bag before sealing, FigureX).
Circle _
_Circle number of location swabbed and label with GMT (dd/hh:mm, FigureX).
If _
_If swab is contaminated by touching a surface other than the designated location on the label,
Label _
_Label NanoRacks Swab Kit with a large, "X" and move on to the next location. Notify POIC of NanoRacks Swab Kit S/N that was contaminated
6. _
_6. Repeat step 2 to step 6 for all 15 locations listed on the NanoRacks Swab Kit label (or reference table below).
NOTE:
An _
_NOTE: _
_An additional large Ziplock Bag is provided (stowed inside the same bag as the NanoRacks Swab Kits) to use per crew preference to separate the used NanoRacks Swab Kits from the clean (unused) NanoRacks Swab Kits for crew efficiency during sampling.
_
### ISS Crew
Swabbing was conducted during Expedition 39. The crew included NASA astronauts Steve Swanson and Rick Mastracchio and Russian cosmonauts Oleg Artemyev, Alexander Skvortsov, and Mikhail Tyurin. Japan Aerospace Exploration Agency (JAXA) astronaut Koichi Wakata was the commander for this expedition, and is the astronaut who performed the swabbing.
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###Comparison of ISS surfaces to analogous surfaces in homes on Earth and human belly buttons
The sequences and associated metadata from a 40-home pilot study for the Wildlife of Our Homes Project are available for download from Figshare \cite{885e3742-e0c3-4719-a6a8-dba9930a33ca}. These were used in a combined analysis with the ISS sequences presented here. Because the sequences from the three projects are not all the same lengths, each dataset was independently analyzed using a closed-reference OTU-picking approach, and the resultant biom tables were merged with the merge\_otu\_tables.py script.