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Jenna M. Lang edited Methods.md
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\cite{Caporaso_2012}.
##Bioinformatic Analysis
Unless otherwise noted, all microbial community analyses were conducted using the QIIME workflow version 1.8 or
R. R \cite{R} All python scripts referred to are components of QIIME \cite{Caporaso_2010}.
###Demultiplex and QC.
An in-house script was used to assign sequences to samples, using dual-index barcoding. This script is available on github (https://github.com/gjospin/Demul_trim_prep)
###OTU assignment and QC
The number of high-quality sequences per sample ranged from 26830 to 77845 (see Table
X). 1). The pick\_open\_reference\_otus.py script was used to cluster sequences at 97% similarity. Taxonomy was assigned to each cluster by comparing a representative sequence from each cluster to the gg\_13\_8\_otus reference taxonomy provided by the Greengenes Database Consortium (http://greengenes.secondgenome.com.)
No chimeric Chimeric sequences were identified using the identify\_chimeric\_seqs.py
script and there were no singleton OTUs (probably should verify these things). script. All subsequent beta diversity analyses (comparisons across samples) were performed with all samples rarefied to 26830 sequences.
###Comparison of ISS surfaces to analogous surfaces in homes on Earth and human belly buttons
The sequences and associated metadata from a 40-home pilot study for the Wildlife of Our Homes Project are available for download from Figshare \cite{885e3742-e0c3-4719-a6a8-dba9930a33ca}. These were used in a combined analysis with the ISS sequences presented here. Because the sequences from the three projects are not all the same lengths, each dataset was independently analyzed using a closed-reference OTU-picking approach, and the resultant biom tables were merged with the merge\_otu\_tables.py script.