deletions | additions       diff --git a/Methods.md b/Methods.md  index fbfa473..bd9bfb5 100644  --- a/Methods.md  +++ b/Methods.md 
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16S rRNA gene (515F-806R) was amplified with region-specific primers that included   the Illumina flowcell adapter sequences and a twelve base barcode sequence. Each 25 ul   PCR reaction contained the following: 12 ul of PCR water certified DNA-free (MO BIO),   10 ul of 1x 5 Prime HotMasterMix (5 Prime), 1 ul of Forward Primer (5uM (5 uM  concentration, 200 pM final), 1 ul of Golay Barcode Tagged Reverse Primer (5uM concentration, 200pM   final), and 1 ul of template DNA. The conditions for PCR were as follows: 94°C for 3   minutes to denature the DNA, with 35 cycles at 94 °C for 45 s, 50 °C for 60 s, and 72 °C