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#General notes on bacterial systematics.  In order to identify to which organism a 16S rDNA sequence belongs, as well as to provide an evolutionary context for your organism of interest, we recommend building a phylogenetic tree (see Section X). Here we will briefly discuss a few terms that will make interpretation of Building  the phylogenetic treea simple task. Interpreting a phylogenetic tree  is much like navigating in a Terminal window, in that you can probably get by with a few basic concepts, but you the easy part. Intelligent interpretation of the tree  will need require an investment of time, similar to the investment required  to invest some time in learning them learn the basics of UNIX. Fortunately, there are a number of resources available for this purpose. We recommend this online tutorial (http://evolution.berkeley.edu/evolibrary/article/phylogenetics_02) or this very nice paper by Baldauf  http://evolution.berkeley.edu/evolibrary/article/phylogenetics_02  this paper: http://research.cs.queensu.ca/home/cisc875/faint.pdf  As with UNIX, we will list some basic terms with which you should make yourself familiar before attempting to make sense of your tree.  ##Branch  All of the organisms in a tree are at the tips of brances (that's why they are sometimes referred to as leaves.) All of the branches show the way in which organisms are connected (related) to each other.  ##Node  This is These are the points  where branches connect.  ##Clade  A clade is a group of organism that  ##Bootstrap support  This is a number, usually expressed on a scale of 0-100, but occasionally on a scale of 0-1, that provides an estimate of the confidence in a node.  ##Monophyly