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Madison edited Organism Identification using 16s rRNA gene sequence.md
almost 10 years ago
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If you have relatively ambiguous identification results (e.g. you think you have some sort of Lysinibacillus but aren't sure which species) it could be worthwhile to perform an alignment of your 16S sequence with those from genomes already in Genbank.
##Align 16S Sequences using Align Sequences Nucleotide BLAST
First locate the 16S sequences of the genome you'd like to compare to, in FigureX, we searched the NCBI Nucleotide database for
"lysinibacillus fusiformis 16S gene ZB2". "Tatumella 16s gene".
http://www.ncbi.nlm.nih.gov/nuccore/