this is for holding javascript data
David Coil edited Library Preparation and Sequencing .md
almost 10 years ago
Commit id: e14ef71601260f0ca60d6693cedbe53bab1c7055
deletions | additions
diff --git a/Library Preparation and Sequencing .md b/Library Preparation and Sequencing .md
index 43b0119..081d011 100644
--- a/Library Preparation and Sequencing .md
+++ b/Library Preparation and Sequencing .md
...
#Library Preparation and Sequencing
##Library Preparation
The first choice in library preparation is whether to do the library prep yourself, or to have the library made by your sequencing provider. The economics of this decision are usually dependent on the number of samples involved. For example an Illumina TruSeq library prep kit costs around $2600 for 48 samples. That's far cheaper than the $150 to $300 that a typical sequencing provider would charge per sample. However, if you're only preparing a couple of samples there's no reason to buy an entire kit. The requisite time and ancillary consumables and equipment must also be taken into
account. account (see . Most sequencing facilities offer library preparation services.
##Kit Options
Whether you chose to make libraries yourself, or use a provider the next major choice is of the type of kit. The two major different choices with Illumina kits are the Nextera transposase-based kits or the TruSeq kits (with or without PCR). These kits are available from Illumina, but there are also comparable options from other vendors (e.g. New England Biolabs and Kappa Bioscience). The pros and cons of each type of kit are listed below: