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Jonathan A. Eisen edited General notes on molecular and microbiology.md
almost 10 years ago
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1. What type of organism do you want to isolate?
2. Are there types of organisms (e.g., pathogens) that you would prefer not to isolate? For example, swabbing people and growing samples on blood agar at \(37\,^{\circ}\mathrm{C}\) is probably not advised in most cases.
(THIS NEEDS MORE EXPLANATION - WHY IS THIS NOT ADVISED)
3. How much time is available for growth and isolation?
+
growth rates differ both between organisms (e.g., species 1 versus species 2) and also in different conditions for the same organisms (e.g., species 1 at \(20\,^{\circ}\mathrm{C}\) vs. \(37\,^{\circ}\mathrm{C}\))
+ many bacteria will grow faster at higher temperatures, but at some point, a threshhold will be reached and many species won't grow
(PLEASE REMOVE ALL CONTRACTIONS LIKE WON'T)
+ you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow
5. What types of equipment are available to you?