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1. What type of organism do you want to isolate?  2. Are there types of organisms (e.g., pathogens) that you would prefer not to isolate? For example, swabbing people and growing samples on blood agar at 37\(10\,^{\circ}\mathrm{C}\)C \(37\,^{\circ}\mathrm{C}\)  is probably not advised in most cases.   3. How much time is available for growth and isolation?