deletions | additions       diff --git a/General notes on molecular and microbiology.md b/General notes on molecular and microbiology.md  index 7c9af17..4e31c39 100644  --- a/General notes on molecular and microbiology.md  +++ b/General notes on molecular and microbiology.md 
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1. What type of organism do you want to isolate?  2. Are there types of organisms (e.g., pathogens) that you would prefer not to isolate? For example, swabbing people and growing samples on blood agar at \(37\,^{\circ}\mathrm{C}\) is probably not advised will often result  in most cases. (THIS NEEDS MORE EXPLANATION - WHY IS THIS NOT ADVISED) the isolation of pathogens.    3. How much time is available for growth and isolation?  + growth rates differ both between organisms (e.g., species 1 versus species 2) and also in different conditions for the same organisms (e.g., species 1 at \(20\,^{\circ}\mathrm{C}\) vs. \(37\,^{\circ}\mathrm{C}\)) 
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+ you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow    4. What types of equipment are available to you?  + if something grows most happily at \(37\,^{\circ}\mathrm{C}\), then you will need to have an incubator and shaker available at that temperature (EXPLAIN WHY YOU NEED A SHAKER?) temperature.  For our previous work we have simply used a rich media such as lysogeny broth (LB) and growth at either room temperature or \(37\,^{\circ}\mathrm{C}\).