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This figure shows the estimated materials (i.e. without labor) cost of performing a genome sequencing project with this workflow. The "Best Case" shows the marginal cost of sequencing one genome in a case where you are multiplexing 48 samples, and have the appropriate kits and reagents on hand. The "Worst Case" shows the cost of doing a single genome, with no multiplexing, in a lab where every reagent needed to be purchased new and was not used for anything else. Specific assumptions are as follows;   ¹  This assumes the purchase of a standard DNA extraction kit, good for 100 samples.   ²This assumes purchase of a standard 200U PCR reagent kit.   ³PCR cleanup can be performed in a number of ways; gel extraction, beads, or columns for example. Here we assume purchase of a standard column-based kit.   ⁴Sanger sequencing cost is given as the price per reaction ($7 at our sequencing facility), times the forward and reverse reactions.