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Jenna M. Lang edited Building a 16S rDNA Phylogenetic Tree.md
almost 10 years ago
Commit id: bcc583a228cc0657704f2041f08fe74037d13f3a
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In some rare cases the tree generated by RDP will be sufficient, other times it may fail to load, crash, or be impossible to resolve detail. We recommend instead, downloading the alignment and building the tree in FastTree.
Building ##Clean up the
Tree with FastTree RDP taxon names
Download the alignment you just created in RDP
-Click on download, use the default settings (fasta, aligned, uncorrected )
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$ perl /Users/microBEnet/Desktop/RDPcleanup.pl -i /Users/microBEnet/Desktop/newTatumella.fa -o TatumellaRAST_RDP.fa
##Building the Tree with FastTree
Go to
http://www.microbesonline.org/fasttree/#Install
and download the FastTree.c program by right clicking on it and saving the link
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The alignment file will be the output file of the cleanup script
And the tree_file output name should include a path to where you want it to be located. The output name should also end in .tre in order to be recognized by Dendroscope
Viewing ##Viewing the Tree
in Dendroscope
Download and install Dendroscope
http://ab.inf.uni-tuebingen.de/software/dendroscope/