Jenna M. Lang edited Building a 16S rDNA Phylogenetic Tree.md  almost 10 years ago

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In some rare cases the tree generated by RDP will be sufficient, other times it may fail to load, crash, or be impossible to resolve detail. We recommend instead, downloading the alignment and building the tree in FastTree.  Building ##Clean up  the Tree with FastTree RDP taxon names  Download the alignment you just created in RDP  -Click on download, use the default settings (fasta, aligned, uncorrected ) 

$ perl /Users/microBEnet/Desktop/RDPcleanup.pl -i /Users/microBEnet/Desktop/newTatumella.fa -o TatumellaRAST_RDP.fa  ##Building the Tree with  FastTree Go to  http://www.microbesonline.org/fasttree/#Install  and download the FastTree.c program by right clicking on it and saving the link 

The alignment file will be the output file of the cleanup script  And the tree_file output name should include a path to where you want it to be located. The output name should also end in .tre in order to be recognized by Dendroscope  Viewing ##Viewing  the Tree in Dendroscope  Download and install Dendroscope  http://ab.inf.uni-tuebingen.de/software/dendroscope/