Jenna M. Lang edited General notes on bacterial systematics.md  almost 10 years ago

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***Monophyletic clade*** If every representative of a species is found in one single clade, and no representatives of that species are found outside of that clade, then that is a monophyletic clade. The concept of monophyly is important because all species should represent monophyletic groupings. If all species are not found in a monophyletic group, then taxonomic revision of that species is in order. Unfortunately for bacterial species, taxonomic revision is frequently in order.  ***Rooting/Outgroup Selection*** The root is at the base of the tree, and is determined by the designation of an outgroup. The outgroup is an organism that you are confident is most distantly related to everything else in your tree. Outgroup selection is not a trivial task, but to simplify it here, we suggest always using an Archaeal 16S rDNA  sequence as your outgroup.