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David Coil edited Data Submission.md
about 9 years ago
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##Submitting contigs only
Use this section if submitting only contigs, presumably in FASTA format
Navigate to
http://www.ncbi.nlm.nih.gov. [http://www.ncbi.nlm.nih.gov](http://www.ncbi.nlm.nih.gov). Create an account and/or login. Then, create a BioProject at NCBI by navigating to
https://submit.ncbi.nlm.nih.gov/subs/bioproject/ [https://submit.ncbi.nlm.nih.gov/subs/bioproject/](https://submit.ncbi.nlm.nih.gov/subs/bioproject/) and clicking on "New submission." Fill in the personal information for the submitter.
Below, in italics, are the responses that we typically give for a genome sequencing project.
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##Create a Whole Genome Shotgun (WGS) Submission
Navigate to
https://submit.ncbi.nlm.nih.gov/subs/wgs/ [https://submit.ncbi.nlm.nih.gov/subs/wgs/](https://submit.ncbi.nlm.nih.gov/subs/wgs/)
Click on the New Submission button at the top
Submitter
-fill in your own information
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**Create a SBT template**
Create a SBT template file at NCBI
http://www.ncbi.nlm.nih.gov/WebSub/template.cgi [http://www.ncbi.nlm.nih.gov/WebSub/template.cgi](http://www.ncbi.nlm.nih.gov/WebSub/template.cgi)
The BioProject # is the Bioproject ID starting with "PRJNA" which you received above, BioSample can be left blank
When you click create the template, it will automatically download to your computer as template.sbt. We recommend immediately renaming the file to the appropriate project.
**Tbl2asn**
Download the tbl2asn program from
ftp://ftp.ncbi.nih.gov/toolbox/ncbi\_tools/converters/by\_program/tbl2asn/ [ftp://ftp.ncbi.nih.gov/toolbox/ncbi\_tools/converters/by\_program/tbl2asn/](ftp://ftp.ncbi.nih.gov/toolbox/ncbi\_tools/converters/by\_program/tbl2asn/)
If you are using Safari, a window will pop up asking for login information, just choose guest and unzip the version of the program that is compatible with your operating system. Other browsers will take you to a page with a lot of tbl2asn programs, download the one compatible with your operating system.
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Go to:
https://www.ebi.ac.uk/ena/about/sra_submissions [https://www.ebi.ac.uk/ena/about/sra_submissions](https://www.ebi.ac.uk/ena/about/sra_submissions)
And create an account