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2. Are there types of organisms (e.g., pathogens) that you would prefer not to isolate? For example, swabbing people and growing samples on blood agar at 37 deg C will often result in the isolation of pathogens.    3. How much time is available for growth and isolation?  + growth rates differ both between organisms (e.g., species 1 versus species 2) and also in different conditions for the same organisms (e.g., species 1 at 20 deg C vs. 37 deg C C)  + for many microbes there is an "optimal growth temperature"(OGT temperature" (OGT  - the temperature at which it grows best) but the OGT varies between species + you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow    4. What types of equipment are available to you?