David Coil edited 16S rDNA Sequencing and Analysis (Organism Identification).md  almost 10 years ago

Commit id: a809db3074133d760b6e47e978fa9cfd1a0fc8b6

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###Download/Install  1. Create a new folder called Sanger_seq somewhere on your computer  2. Download the script from https://github.com/gjospin/scripts  3. Download a zip of all of the files, unzip it and move the merge_sanger_16s.pl merge\_sanger\_16s.pl  script to the Sanger_seq folder ###MUSCLE  In order to run this script you will need to download MUSCLE (REF) from here: http://www.drive5.com/muscle/downloads.htm. Use the Archive Utility to open the file, change the name of the executable file from something like "muscle3.8.31_i86darwin64" to "muscle," and move it into your Applications folder.