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##Interpreting the results  Depending on the quality of the Sanger sequencing and the particular bacteria sequenced, the BLAST results can range from definitive to relatively uninformative. Examples of both are discussed below.  1. In some cases it is not necessary to build a phylogenetic tree for further identification. If all of the top hits are the same species (or end in sp.), have e-values of 0.0, perfect good  query coverage, and 99% to 100% identity you can proceed to "Using GOLD". 2. In other cases the results are much more ambiguous. The results may show more than 99% identity to multiple species within multiple genera. In this case, proceed to section 11 "Building a 16S rDNA Phylogenetic Tree", before using GOLD.  ##Using GOLD (the Genomes Online Database)