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4. What types of equipment are available to you?  + if an organism grows most happily at 37 °C, then you will need to have an incubator and shaker available at that temperature.  For our previous work we have simply used a rich media such as lysogeny broth (LB) and growth at either room temperature (~25 °C) or 37 °C. For some basic information on media preparation and agar plates, we recommend the following resource: [http://teach.genetics.utah.edu/content/gsl/html/agar.html](http://teach.genetics.utah.edu/content/gsl/html/agar.html)