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The goal of this section is to obtain a 16S alignment from RDP that can be used to build a tree. This procedure has the added benefit of providing an independent verification of the taxonomic assignment of your sequence based on the BLAST results.  1. Go to rdp.cme.msu.edu [http://rdp.cme.msu.edu](http://rdp.cme.msu.edu)  2. Create an account   3. Click on my RDP/login   4. Upload the fasta file containing your 16s 16S  sequence 5. Assign it a group name (this is what the program will label your sequence/organism). Choose this carefully since that will be the name on the final tree.   6. Click the "+" next to the sequence, to add it to your cart  7. Click on CLASSIFIER at the top of the page