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#General notes on molecular and microbiology  This section workflow  assumes basic knowledge of molecular biology and sterile technique. The starting pointfor this workflow  is the collection of microbes from a surface with a cotton swab. Here we We will  cover the steps necessary to take a sample through plating, dilution streaking, overnight growth, creating a glycerol stock, 16s rDNA PCR, and preparation for Sanger sequencing to determine the identity of your bacterial isolate. Throughout this the "Isolation"  section we refer to "agar" and "culture media". The choice of media will depend on the goals of the particular project. Some factors to consider when selecting media and conditions for growth include: 1. What type of organism do you want to isolate? 
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  3. How much time is available for growth and isolation?  + things many bacteria  will grow faster at higher temperatures, but at some point, a threshhold will be reached and many species won't grow + you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow    5. What types of equipment are available to you?  + if something grows most happily at \(37\,^{\circ}\mathrm{C}\), then you will need to have an incubator and shaker available at that temperature  For our previous work we have simply used a rich growth media such as lysogeny broth (LB) and growth at either room temperature or \(37\,^{\circ}\mathrm{C}\).