deletions | additions       diff --git a/16S rDNA Sequencing and Analysis (Organism Identification).md b/16S rDNA Sequencing and Analysis (Organism Identification).md  index b91d153..5e5034c 100644  --- a/16S rDNA Sequencing and Analysis (Organism Identification).md  +++ b/16S rDNA Sequencing and Analysis (Organism Identification).md 
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We have also created a script that will do all of these steps automatically, but does not allow you to adjust any of the parameters The choice of our script (easy, little control) versus SeqTrace (more complex, more control) will depend on the user and the project.   ##SeqTrace ##Install and run SeqTrace  Download the program from  https://code.google.com/p/seqtrace/downloads/list 
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To install SeqTrace on a Mac you will need to download the PyGTK package from OSX.   http://sourceforge.net/projects/macpkg/files/PyGTK/2.24.0/PyGTK.pkg/download  Confirm that you have Python version 2.7x or earlier. 2.x.  You can do this by typing typing:  - python --version    You should see something that looks like "Python 2.6.9"  If not, you see Python 3.x,  seek help to invoke an earlier version directly. http://www.python.org/download/releases/  After downloading and unpacking the program, SeqTrace is ready to go. SeqTrace must be launched from a Terminal window. For a refresher or introduction to the Terminal see section II. Move SeqTrace to your Applications folder.  
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./Applications/seqtrace-0.9.0/seqtrace.py  This syntax will only work if the SeqTrace folder’s name is seqtrace-0.9.0, if you saved it under a different name you will need to replace seqtrace-0.9.0 with whatever you named your the name of that  folder This will launch SeqTrace from the terminal in a Python shell, shell;  you will need to keep the terminal window open while you are using the program. SeqTrace provides excellent directions for using the program at https://code.google.com/p/seqtrace/wiki/WorkingWithProjects  Edit ##Edit  and Create a Consensus Sequence For this workflow we have found that the following is the simplest way to edit and create a consensus sequence from a forward and reverse read in SeqTrace.  1. Create a new project (File > New Project)