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Madison edited 16S rDNA Sequencing and Analysis (Organism Identification).md
almost 10 years ago
Commit id: 905b401748d212a4f725a9b7bb9fe2029e763fe9
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Use the drop down menus to set it to convert .abi files to .fastq. Upload a file and convert it. The converted file will save to your downloads folder under the name sample.fastq. If you are working with a lot of reads we recommend immediately renaming the files to match the original abi file name to avoid confusion.
###Edit and Create a Consensus Sequence
Once all of your files are in fastq format, move all of them to the Sanger\_seq folder in which you saved the merge\_sanger\_16s.pl script. Use the terminal to navigate to within this
folder. Then, type: folder by typing:
cd Desktop/Sanger_seq