David Coil edited Building a 16S rDNA Phylogenetic Tree.md  almost 10 years ago

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Once the tree is visible, the first step is to re-root the tree to the outgroup. Expand the tree by clicking the expansion button (labeled in Figure X), then scroll through the tree to locate the outgroup. Click on the beginning of the taxa name, to select it, and reroot the tree by going to edit and selecting re-root.  We recommend viewing the tree as a phylogram which can be accomplished by clicking on the phylogram button (labeled in Figure X). From this tree it should be possible to determine the phylogenetic placement of the candidate sequence, and in some cases to give it a name with more certainty than a simple BLAST search. Below are examples of a relatively informative tree and a relatively uninformative tree:  TI    In tree shown above (genus Brachybacterium), our sample of interest from an assembly is "Brachybacterium muris UCD-AY4" \cite{Lo_2013}. \cite{Lo\_2013}.  It falls within a clade where every named member has the same name "Brachybacterium muris", and this name does not occur elsewhere on the tree. Hence, we were confident enough to name our sample as that species. In other words, this sequence falls within a well-supported(note 0.999 bootstrap support,)  monophyletic clade of _Brachybacterium muris_.   In UPDATE THIS FOR TATUMELLAIn  the tree shown above (genus Microbacterium) our species of interest is Microbacterium sp. str. UCD-TDU \cite{Bendiks_2013}. In contrast to the Brachybacterium example, here our species falls within a poorly defined clade containing multiple species. In this case we did not assign a species name to this isolate.