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3. how much time is available for growth and isolation?  4. + things will grow faster at higher temperatures, but at some point, a threshhold will be reached and many will die  + you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow  4. 5.  what types of equipment are available to you? + if something grows most happily at 37deg, then you will need to have an incubator and shaker available at that temperature  4.   For our previous work we have simply used a rich growth media such as lysogeny broth (LB) (REF)