deletions | additions       diff --git a/General notes on molecular and microbiology.md b/General notes on molecular and microbiology.md  index d7412a2..7c9af17 100644  --- a/General notes on molecular and microbiology.md  +++ b/General notes on molecular and microbiology.md 
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  3. How much time is available for growth and isolation?  + growth rates differ both between organisms (e.g., species 1 versus species 2) and also in different conditions for the same organisms (e.g., species 1 at \(20\,^{\circ}\mathrm{C}\) vs. \(37\,^{\circ}\mathrm{C}\))  + for  many bacteria will grow faster microbes there is an "optimal growth temperature"(OGT - the temperature  at higher temperatures, which it grows best)  but at some point, a threshhold will be reached and many the OGT varies between  specieswon't grow (PLEASE REMOVE ALL CONTRACTIONS LIKE WON'T)  + you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow    5. 4.  What types of equipment are available to you? + if something grows most happily at \(37\,^{\circ}\mathrm{C}\), then you will need to have an incubator and shaker available at that temperature (EXPLAIN WHY YOU NEED A SHAKER?)  For our previous work we have simply used a rich media such as lysogeny broth (LB) and growth at either room temperature or \(37\,^{\circ}\mathrm{C}\).