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1. What type of organism do you want to isolate?  2. Are there types of organisms (e.g., pathogens) that you would prefer not to isolate? For example, swabbing people and growing samples on blood agar at \(37\,^{\circ}\mathrm{C}\) 37 deg C  will often result in the isolation of pathogens.   3. How much time is available for growth and isolation?  + growth rates differ both between organisms (e.g., species 1 versus species 2) and also in different conditions for the same organisms (e.g., species 1 at \(20\,^{\circ}\mathrm{C}\) 20 deg C  vs. \(37\,^{\circ}\mathrm{C}\)) 37 deg C  + for many microbes there is an "optimal growth temperature"(OGT - the temperature at which it grows best) but the OGT varies between species  + you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow    4. What types of equipment are available to you?  + if something grows most happily at \(37\,^{\circ}\mathrm{C}\), 37 deg C,  then you will need to have an incubator and shaker available at that temperature. For our previous work we have simply used a rich media such as lysogeny broth (LB) and growth at either room temperature or \(37\,^{\circ}\mathrm{C}\). 37 deg C.