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Feel free to add notes/critiques of the rocketship/summary figure here  The Figure above illustrates all of the steps of the workflow. A short desescription of each step and its equivalent ??? are listed below.  **Culture and Isolation**-Here Here we cover the steps necessary to take a sample through plating, dilution streaking, overnight growth, creating a glycerol stock, 16s PCR and preparation for Sanger sequencing.   We assume a starting point of wanting to isolate an organism from a particular environment and needing to identify it. Users starting with a known organism should proceed to "Library Preparation and Sequencing”.  Culture and Isolation covers steps 5 (Isolation) through 6.3 (PCR reaction)