this is for holding javascript data
David Coil edited figures/workflow2/caption.md
almost 10 years ago
Commit id: 7df18de25424ba8f7bce66b7eec9832c2e41c28f
deletions | additions
diff --git a/figures/workflow2/caption.md b/figures/workflow2/caption.md
index fdf1f3e..38e53b9 100644
--- a/figures/workflow2/caption.md
+++ b/figures/workflow2/caption.md
...
**Alignment**-Upon receiving Sanger reads from a sequencing facility, it is necessary to quality trim the reads, reverse complement the sequences, align the reads and generate a consensus sequence. This is easiest to do visually via a chromatogram allowing the user to see the trace and process the sequences manually.
**I.D. Species**-In a classroom or undergraduate research setting the researchers may not have a particular bacterial species in mind. In this case it is necessary to screen the 16S Sanger sequencing results for possible genome project candidates. We recommend starting with the BLAST results, then continuing onto the Genomes Online Database (GOLD), and simply Google searching. In many cases it will be handy to first build a phylogenetic tree to aid in identification since the
16S BLAST results may not be sufficiently informative.
**DNA Extraction**-In this step, DNA is extracted from the species of interest.