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3. how much time is available for growth and isolation?  \t  things will grow faster at higher temperatures, but at some point, a threshhold will be reached and many will die 3.2.  you will be able to isolate a greater diversity of organisms if you allow a long time for slow-growing things to grow   4. what types of equipment are available to you?  4.1.if if  something grows most happily at 37deg, then you will need to have an incubator and shaker available at that temperature For our previous work we have simply used a rich growth media such as lysogeny broth (LB) (REF)