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David Coil edited Data Submission.md
over 9 years ago
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+ Isolation source
+ Files
+ Select _We have files for traditional split contigs OR gapped sequences_
+ Select _
**FIGURE THIS OUT** "FASTA", upload the files
+
"Bacteria is available from" _If Select "No" for the
bacteria is available in question about scaffolds
+ "Is any sequence a
culture collection, feel free to indicate where. We recommend submission of sequenced strains complete chromosome?" _No_
+ "Does any sequence belong to a
culture collection if possible._
+ Source DNA is available from-_See above_ plasmid" _No_
-Check the box below to annotate this prokaryotic genome in the NCBI prokaryotic annotation pipeline before being released. This will allow NCBI to use their PGAAP pipeline to annotate the genome, and this annotation will be automatically attached to the project.
**Files**
CHECK ALL OF THIS
+ Click on “We have files for contigs”
+ Did you assemble the contigs or other components into scaffolds and/or chromosomes? _Yes_
+ Do you have unplaced scaffolds (scaffolds without chromosome or plasmid information)? _Yes_-upload AGP file
+ Did you assemble chromosomes, plasmids and/or unlocalized scaffolds? _No_
+ Do you have sequence files for scaffolds and/or chromosomes and/or plasmids? _No_
Click "Submit" and you're done! You will receive a series of e-mails from NCBI confirming your submission and notifying you of any problems. Once the submission is pre-processed you'll get an Accession Number. Note however that the data will not be released until final processing. The Accession Number is not acceptable for publication until after the final release of the data.
Potential problems with data submission:
...
cat test.fa | awk '{print (NR%2==1) ? ">contigs_" ++i : $0}' > test2.fa
##Submitting scaffolds
Only _**Only use this section if you are submitting
scaffolds, scaffolds**, in most cases assembly with A5 will render this step uneccessary. Many of the steps are the same as for submitting contigs, only the diferences are shown
here. here._
Before submitting your scaffolded genome, you will need to have available 4-5 files which are listed below.