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After growth to visible colonies, repeat the dilution streaking to help ensure purity of the culture. Some organisms will only grow in tight association with others, and a mixed culture will prove difficult to work with and very difficult to sequence/assemble.   ##Liquid Culture  After the second dilution streaking, a liquid culture is needed both for long-term storage and for DNA extraction. Pick a single colony from each dilution streak plate into 5mls 5 mls  of culture media and grow for 1-3 days until cloudy. Once the liquid culture is ready, prepare a 10% final concentration glycerol stock for long-term storage at -80 deg C from 2mL 2 ml  of the sample.