David Coil edited Genome Assembly and Annotation.md  almost 10 years ago

Commit id: 71281523fcedd46c128cdf775ccbbf31a4418863

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###Running A5  Once you have opened the terminal navigate to the folder you just created because A5 will output the files your location when you call the program. In this example I created the folder on the desktop and named it a5_ouput so the syntax for navigating to the folder is   $ cd Desktop/a5_output/ Once there the easiest way to run the program is to drag and drop the a5 pipeline into the terminal. Open the bin folder located in the downloaded folder. Drag the file labeled a5_pipeline.pl into the terminal   __add arrow to picture___  then drag in the input file(s) (the paired end read files). Finally name the output files   the final syntax will read   $ a5_pipeline.pl read_1.fastq read_2.fastq mygenome /Users/Madison/Desktop/a5_miseq_macOS_20140113/bin/a5_pipeline.pl is the pipeline and its location  /Users/Madison/Desktop/a5_miseq_macOS_20140113/example/phiX_p1.fastq is the first paired end read