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Confirm that you have Python version 2.x. You can do this by typing:  -  python --version You should see something that looks like "Python 2.6.9" If you see Python 3.x, seek help to invoke an earlier version directly.  http://www.python.org/download/releases/  After downloading and unpacking the program, SeqTrace is ready to go. SeqTrace must be launched from a Terminal window. For a refresher or introduction to the Terminal see section II. Move SeqTrace to your Applications folder.   Open a Terminal window and type type:  -./Applications/seqtrace-0.9.0/seqtrace.py ./Applications/seqtrace-0.9.0/seqtrace.py  This syntax will only work if the SeqTrace folder’s name is seqtrace-0.9.0, if you saved it under a different name you will need to replace seqtrace-0.9.0 with the name of that folder